Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR

by F. Marabita, P. de Candia, A. Torri, J. Tegnér, S. Abrignani, R.L. Rossi
Year:2016

Bibliography

Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR
F. Marabita, P. de Candia, A. Torri, J. Tegnér, S. Abrignani, R.L. Rossi
Briefings Bioinformatics, 204-212, 2016

Abstract

​The high-throughput analysis of microRNAs (miRNAs) circulating within the blood of healthy and diseased individuals is an active area of biomarker research. Whereas quantitative real-time reverse transcription polymerase chain reaction (qPCR)-based methods are widely used, it is yet unresolved how the data should be normalized. Here, we show that a combination of different algorithms results in the identification of candidate reference miRNAs that can be exploited as normalizers, in both discovery and validation phases. Using the methodology considered here, we identify normalizers that are able to reduce nonbiological variation in the data and we present several case studies, to illustrate the relevance in the context of physiological or pathological scenarios. In conclusion, the discovery of stable reference miRNAs from high-throughput studies allows appropriate normalization of focused qPCR assays.

DOI: 10.1093/bib/bbv056

Normalization of circulating microRNA expression data.pdf

Keywords

Normfinder Circulating miRNA GeNorm Normalization qPCR Reference genes
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